yxeK [2018-04-08 17:42:07]

similar to dibenzothiophene sulfone monooxygenase

locus: BSU39520

pI: 5.67

mw: 49.18 kDa

protein length: 441 aa

gene length: 1323 bp

function: sulphur metabolism

product: similar to dibenzothiophene sulfone monooxygenase

essential: no

synonyms

Genomic Context

categories

• [category|SW 2|Metabolism] → [category|SW 2.6|Additional metabolic pathways] → [category|SW 2.6.4|Sulfur metabolism] → [category|SW 2.6.4.3|Conversion of S-(2-succino)cysteine to cysteine]
• [category|SW 2|Metabolism] → [category|SW 2.7|Detoxification reactions]

Gene

Coordinates: 4,060,818 → 4,062,143

Phenotypes of a mutant

• no growth with S-(2-succino)cysteine as the single source of sulfur [pubmed|29626092]

The protein

Protein family

• ntaA/snaA/soxA(dszA) monooxygenase family (according to Swiss-Prot)

Paralogous protein(s)

• [protein|8145952307BC47805A7A9BD39418474358043BFE|CmoJ]

Structure

• [PDB|5TLC] (from B. subtilis WU-S2B 42% identity) [pubmed|28205250]

Expression and Regulation

Operons

• genes: [gene|19E6EF9CCA31440EAC046F8D6CA98B6A08589D58|yxeK]-[gene|EBC2B9107AD50DD8F8174FC0BE4AD90DEE6A7CCA|snaB]-[gene|D96EEDABB73AC66E6AB772431819E7C767DC8B9A|yxeM]-[gene|FFD5F07480A0E5A74852C69C436EE289BA72FC7F|yxeN]-[gene|F8C800CF71D21A1F3265977C4F0EE191D0FFE36A|yxeO]-[gene|C99AA3FDBB561BCED60915078DC746690C071753|sndB]-[gene|7DDB283173267AA106DC277787AAAB4F4F9975F4|yxeQ]
  description: [Pubmed|10746760]

  sigma factors

  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA]: sigma factor, [Pubmed|16513748], in [regulon|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA regulon]

  regulatory mechanism

  • [protein|50930C56C27D22715620A350220E3C56ADB41020|CymR]: repression, [Pubmed|16513748], in [regulon|50930C56C27D22715620A350220E3C56ADB41020|CymR regulon]

  regulation

  • repressed in the presence of cysteine ([protein|50930C56C27D22715620A350220E3C56ADB41020|CymR]) [Pubmed|16513748]

  additional information

  • the amount of the mRNA is substantially decreased upon depletion of [gene|872CCB5A49C9000BD95E4B0472556D5F60F7D7A4|RNase Y] [PubMed|21815947]

Biological materials

Mutant

• MGNA-B709 (yxeK::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1708 NBRP B. subtilis, Japan]
• BKE39520 (Δ[gene|19E6EF9CCA31440EAC046F8D6CA98B6A08589D58|yxeK]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE39520 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGGCGATACTCCTTTTAT, downstream forward: _UP4_TATGCAAAGTAAGGAGGAAC
• BKK39520 (Δ[gene|19E6EF9CCA31440EAC046F8D6CA98B6A08589D58|yxeK]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK39520 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGGCGATACTCCTTTTAT, downstream forward: _UP4_TATGCAAAGTAAGGAGGAAC

References

• 10746760,16513748,21815947,28205250,29497411,29626092